Towards Automatic Electron Tomography for Rod-Shaped Specimen
نویسندگان
چکیده
منابع مشابه
Automated electron microscope tomography using robust prediction of specimen movements.
A new method was developed to acquire images automatically at a series of specimen tilts, as required for tomographic reconstruction. The method uses changes in specimen position at previous tilt angles to predict the position at the current tilt angle. Actual measurement of the position or focus is skipped if the statistical error of the prediction is low enough. This method allows a tilt seri...
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were “unknown agents.” For the sake of objectivity, we based our assumption on the aggregate of information for known pathogens rather than on “expert opinion.” Interestingly, however, the Council of Science and Technology’s “expert opinion” of the percentage of diarrheal illness due to foodborne transmission was 35% (1), nearly identical to the figure we developed. As noted in our article, pat...
متن کاملSpecimen collection for electron microscopy.
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In general the SEM sample preparation techniques are more or less similar to the metallographic sample preparation technique for optical microscopy, because both the microscopes reveals the surface topography of sample. In SEM a focussed beam of electrons scans over the specimen surface and the electrons emitted from the sample surface controls the brightness of the CRT spot which also scans th...
متن کاملMarkov random field based automatic image alignment for electron tomography.
We present a method for automatic full-precision alignment of the images in a tomographic tilt series. Full-precision automatic alignment of cryo electron microscopy images has remained a difficult challenge to date, due to the limited electron dose and low image contrast. These facts lead to poor signal to noise ratio (SNR) in the images, which causes automatic feature trackers to generate err...
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ژورنال
عنوان ژورنال: e-Journal of Surface Science and Nanotechnology
سال: 2010
ISSN: 1348-0391
DOI: 10.1380/ejssnt.2010.178